Journal: Small (Weinheim an der Bergstrasse, Germany)

Article Title: Cellular association and assembly of a multistage delivery system.

doi: 10.1002/smll.201000126

Figure Lengend Snippet: Figure 7. Endothelial targeting with the assembled MDSs. A) HUVECs were incubated with either control IgG fluorescein-labeled MDSs (A) or anti- VEGFR-2 fluorescein antibody-labeled MDSs (B,C) for 2 h at 37 8C (10:1 ratio of particles to cells). C) Antibody-targeted cells are shown at two magnifications,with3Dimagesatthecrosshairsofthehighermagnificationimage(middle).Totheright,lamellopodiaprojectingfromtwocellsmake contactwiththeMDS(63oilimmersionlens;A,B)bar25 mm,C) bars50and7.5 mm;actin:rhodaminephalloidin;nuclei:DRAQ5).D)Flowcytometry dot plot (left) showing microparticle light scatter and a histogram (right) showing microparticle fluorescence before and after conjugation with fluorescentantibody. E) Bar graph (top) showingthe relative association of the MDSswith HUVEC andHMVEC endothelial cells conjugated with either control IgG or anti-PECAM-specific antibody. Below are representative flow cytometry dot plots showing the increase in side scatter in HUVECs after incubation with targeted (anti-PECAM) MDSs.

Article Snippet: Antibody conjugation: Anti-PECAM antibody was obtained from Sigma, while anti-VEGF R2/KDR (vascular endothelial growth factor receptor) and isotype control (IgG1) fluorescein-conjugated antibodies were purchased from R&D Systems (Minneapolis, MN).

Techniques: Incubation, Control, Labeling, Conjugation Assay, Cytometry

Journal: Frontiers in Immunology

Article Title: Negative Immunomodulatory Effects of Type 2 Porcine Reproductive and Respiratory Syndrome Virus-Induced Interleukin-1 Receptor Antagonist on Porcine Innate and Adaptive Immune Functions

doi: 10.3389/fimmu.2019.00579

Figure Lengend Snippet: PRRSV-induced IL-1Ra was not involved in suppression of IFN-γ-producing T lymphocytes in both (A) polyclonal and (B) recalled CSFV responses. The supernatants obtained from type 2 PRRSV or mock (MARC-145 cell lysate) were pretreated with anti-IL-1Ra Ab for 2 h prior to addition into the culture. PBL or PBMC were cultured with PHA, CSFV or controls for 48 h, in the presence of the pretreated supernatants. ± indicates presence/absence of indicated treatment within the culture. Data represents mean ± SD from 5 pigs. Statistical significance was analyzed using ANOVA followed by Tukey's test. * indicates significant difference at p < 0.05.

Article Snippet: In some experimental conditions, cells were pre-treated with final concentration of 10 ng/mL polyclonal goat anti-porcine IL-1Ra antibody (R&D system, clone AF780) or polyclonal goat IgG isotype control antibody (R&D system) at 2 h post-inoculation to neutralize PRRSV-induced IL-1Ra which was then cultured for another 22 h. To determine the effect of PRRSV-induced IL-1Ra on MoDC phagocytic activity, the antibody pre-treated MoDC (2 × 10 6 cell) were further incubated with inactivated E. coli -FITC (ThermoFisher Scientific) in complete RPMI at a MoDC: E. coli ratio of 1:50 for 10 min at 37°C.

Techniques: Cell Culture